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Protein samples (20 g) were prepared by adding lithium dodecyl sulfate (LDS) sample buffer (4) and reducing sample agent (10)
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After incubated for the time points described in the figure legends at room temperature, the reactions were arrested by adding the proteoliposome solution to 200 l ice-cold wash buffer and aspirating it through 0.22-m membrane filters (Millipore, MSGVN2210) under vacuum
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Glutamatergic abnormalities in schizophrenia: a review of proton MRS findings
Yanoff 2011
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