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For reducing SDS-PAGE, 2 g of protein was mixed with 3 L of 4 Bolt LDS Sample Buffer (Invitrogen), 1.2 L of 10 Bolt Sample Reducing Agent (Invitrogen), and Milli-Q water to a total volume of 12 L, and the samples were incubated for 10 min at 95 C
Environ Toxicol Pharmacol 74:103312
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Biopreserv Biobank
Y., Zhao, L., Ge, Y
Lehto, S
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