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doi: 10.1093/eurheartj/ehr304 37
One hundred microliter aliquot of tissue homogenate supernatant was first incubated with 50 L KOH (1 mol/L) at 37 C for 30 min to fully hydrolyze the combined carnitine, and 10 L HCl (1 mol/L) was then added to neutralize the solution (sample A)
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The trace metal is Zn, Se, Cr, Cu, Mn, or mixtures thereof
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