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Isolation of polar metabolites For the metabolomics of tissues, 100200 mg of weighed tissue was homogenized in 1 ml of ice-cold 80% methanol with a QIAGEN Tissue Lyser II (three sets of 2 min on maximum speed, with 2 min of rest on dry ice in between sets)

Although many of the genes up- and downregulated by GSTP1 deletion are associated with the RAS/MAPK pathway (Supplementary Table 1), we did not detect a significant difference in RAS/MAPK pathway activation, using a quantitative RAF-1- binding/Ras GTPase ELISA to assess pathway activation in GSTP1 knockdown cells (data not shown)

10.3389/fcimb.2019.00182 115 RyuM

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Cell Stem Cell (2016) 20:23346