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The TGF signaling pathway is involved in a variety of cellular processes, including immune response, extracellular matrix remodeling, angiogenesis, and cell differentiation

Visualization of stained cells was achieved with tyramide-enhanced FITC or rhodamine (1:50 dilution in amplification solution

Total cellular extracts (200 g of protein) were incubated with an anti-IKK- antibody (1 g/mL) in IP buffer overnight at 4 C on a rocker

All these data indicate the close relationship between the metabolism of NO, H 2 S, and ROS, since key enzymes involved in the metabolism of all these molecules are regulated among themselves, which creates a complex signaling network that affects numerous biological processes from the germination, development, senescence, and to fruit ripening

Various signalling cascades, such as PI3K/Akt/mTOR, MAPK/ERK, Wnt/-catenin, the NF-B pathway, JAK/STAT pathway, and TGF- cytokine signalling, collaborate to orchestrate the proliferation, survival, invasion, and metastatic proficiency of malignant cells