Any opacity indicates aggregation (peptide chains clumping) or bacterial contamination
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For lyophilized powder at room temperature, the timeline is more forgiving than you might expect
33 The motor efficacy, safety, and tolerability of RTG therapy have been demonstrated in several 6-month studies involving early and advanced PD patients
Basically, during metabolism, some proteins and fats are broken down by Vitamin B12 so that they could be converted to energy
Given that arguably the most important reactions within neuronal cells is the production of creatine for energy transfer, in limited methylation, due to lower B12, Choline would be "Sacrificed" in order to produce the Methyl group for the conversion of homocysteine to methionine, for the ultimate production of S-Adenosylmethionine, and the production of creatine