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Estimation of protein The protein content was measured as per Bradford, 1976
The fecal homogenate and urine pretreatment for analytes involved simple protein precipitation with methanol
In vivo animal experiments showed that daily administration of NNMTi for 28 days to dietinduced obese (DIO) mice significantly upregulated the expression of lipolysisrelated genes (such as ATGL and HSL) and downregulated lipogenesisrelated genes (such as FAS and ACC), indicating that NNMTi effectively promotes fat breakdown and inhibits fat synthesis
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If youre using a compounding pharmacy, ask for the FDA Registration Number, GMP Certificate, third-party test results, and batch release docs
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