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For instance, in mouse embryonic stem cells, targeted disruption of glucose-6-phosphate dehydrogenase, which is the rate-limiting enzyme in the pentose phosphate pathway, the predominant metabolic pathway for the generation of NADPH (a cofactor of GR), renders cells extremely sensitive to H 2 O 2 -induced cell death 34
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Amplified products were visualized on a 1.5% agarose gel and purified using a kit (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel, Dren, Germany) according to manufacturers instructions, for subsequent Sanger sequencing
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Acta Haematologica, Volume 46, Issue 1 (March, 2009) [xi] - Donnerstag B, Ohlenschlger, Cinatl J, et al
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