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After 18 hours, cells were labelled with 10 nM JFX646 Halo ligand (kind gift from the Lavis lab, Janelia) in optiMEM (Gibco) for 3 minutes, followed by 3x washes in mKRB buffer (140 mM NaCl, 2.8 mM KCl, 2 mM MgCl 2 , 1 mM CaCl 2 , 10 mM HEPES, 10 mM glucose, pH 7.4) at 37C

Get your insurance card, call the member services number on the back, and ask to speak to someone about the specific requirements for bariatric surgery coverage under your plan

While GLP-1 drugs themselves do not directly catabolize muscle, the profound appetite suppression they create can make it extremely easy to stay in a calorie deficit for months

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