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Cells were then loaded for 30 min with 2 mol/l Fluo-4AM (cell-permeant formulation, Thermofisher) in Lockes buffer consisted of 154 mmol/l NaCl, 4 mmol/l NaHCO 3 , 5 mmol/l KCl, 2,3 mmol/l CaCl 2 , 1 mmol/l MgCl 2 , 5 mmol/l glucose, 10 mmol/l Hepes
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Other studies have further supported Mounjaros weight loss effects for diabetic patients [15-17]
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If you need to mix the solution, roll the vial gently between your palms
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