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Nitrous oxide exposure (medical or recreational) can cause acute functional B12 deficiency by inactivating the vitamin, requiring urgent assessment if neurological symptoms develop

coli live in the digestive tracts of people and animals, so finding them can point to sewage reaching the water

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In addition, vitamin B12 is needed to produce myelin and is important for axon and nerve development

This ex vivo model is significant because it uses actual human hair follicles rather than cell cultures or animal models, providing direct evidence of effects on intact human follicular structures