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Hair follicles that were morphologically considered to be in anagen were used in this study
HFF-1 cells maintained in a T-25 cell culture flask (Corning, United States) at 37C in a 5% (v/v) CO 2 atmosphere and harvested at 90% confluence using 0.12% trypsin-EDTA
For these reasons it is generally avoided when other effective options (optimized medication, percutaneous rhizotomy, or Gamma Knife) are available
Holmay MJ, Terpstra M, Coles LD, Mishra U, Ahlskog M, Oz G, et al
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