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The standard reconstitution protocol calls for directing the diluent gently down the side of the vial, allowing it to contact the lyophilized cake gradually rather than blasting it with a direct stream

Data acquisition was done using SerialEM software at 500 nm defocus 44

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Following the original publication describing GLP-1 as a neurotransmitter involved in satiety (13), multiple efforts aimed to determine how neural GLP-1R populations interface with energy homeostasis pathways

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