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Thyroid 15 , 12871290 (2005)

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Gastroenterology & hepatology 16 , 160174 (2019)

Following the manufacturers protocol, we carried out the CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega Italia s.r.l., Milan, Italy)

This delivery method allows the antioxidant to reach the tissues efficiently without being broken down by digestion

When the cells reached approximately 80% confluency, cells were harvested with trypsin and seeded at a density of 2.9 10 5 cells per well in 2.5 ml of culture medium into 6 well flat-bottomed plates (Costar, Corning, USA) and incubated for 24 hr