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We developed a sensitive, high-throughput, scalable system for profiling the cleavage activity of natural and evolved BoNT proteases on thousands of substrates simultaneously by adapting the genetic circuitry of PACE into a pooled activity-enrichment assay

The practical implication is to evaluate medication, sleep, nutrition, metabolic health, substance exposure, inflammation, copper-zinc balance, manganese, antioxidant reserve, methylation and overall function rather than reducing the case to one diagnosis

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Its once-weekly schedule provides convenience for patients needing regular Vitamin D supplementation, especially those who cannot maintain adequate levels through oral or dietary intake

Hasan, T., Nyberg, F., Stephansson, E., Puska, P., Hkkinen, M., Sarna, S., et al

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