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The culture device was placed in a custom-made incubator that maintains a temperature of 37 C, and tissue was maintained half-soaked in sterile, serum-free culture medium (Iscoves Modified Dulbeccos Medium without phenol-red (IMDM, GIBCO) supplemented with 20% KnockOut serum replacement (GIBCO), 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine, 1% non-essential amino acids, 1% HEPES) using a syringe pump
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The clinical trial data is extensive and clear
Equivalent B-1 cells have not been identified in humans but they may have functionally equivalent cells that can colonize the lymphoid tissues and generate natural antibody [45]
PubMed 29945503 Chang CH
These references describe the nutritional roles of the individual components
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