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However, this is a workaround for a pathological condition, not an optimal protocol
The elution was concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column that was pre-equilibrated with 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 1 M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS
Biomaterials 23 , 11211130 (2002)
it has a free thiol group, which is likely to confer particular properties on functional sites of proteins that contain this highly conserved residue
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