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The cells were lysed by sonication (70% amplitude,10-s pulse on/10-s pulse off for 2.5 min)
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Among the enzymes related to GSH metabolism, glutathione S-transferases (GSTs, EC 2.5.1.18) catalyze the binding of various xenobiotics (including numerous pesticides) and their electrophilic metabolites with GSH to produce less toxic and more water-soluble conjugates, so this isoenzyme family has a distinct role in plant detoxification reactions (Marrs 1996, Edwards et al
Peptides enter circulation within 1530 minutes, producing a sharp plasma peak that research shows drops to sub-therapeutic levels within 3648 hours for small peptides under 2,000 daltons
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Reprod Biol 2005;5(1):5-17
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