Consistently, regardless of the initial positions of palmitate either located in the membrane as seen in the cryo-EM structure or placed outside of the membrane, our MD simulations found that the -carboxylate spacer stably inserted into the TM1-TM2 cleft and the 16-carbon palmitic chain is deeply buried in the receptor-lipid interface, contributing multiple strong contacts to stabilize the complexes (Supplementary Fig
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Karagiannis T, Tsapas A, Bekiari E, et al
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doi: 10.2337/diacare.21.9.1414 4 WhitingDRGuariguataLWeilCShawJ
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